Molecular Biology

DNA as the Genetic Material

Campbell Chapters 16, 17, 18 and 20

Lecture topics:

• Experiments leading to DNA as the genetic material
• Structure of DNA
• Connecting genes and proteins.
• DNA replication
• Transcription
• RNA processing
• Translation
• Effects of mutations on gene products
• Genetics of Bacteria and Viruses
• Techniques for examining and manipulating DNA

Labs:

• Human karyotyping – Carolina Biological Types of human Chromosome Abnormalities Biokit
• Determining the length of a DNA sequence through gel electrophoresis (Modern Biology experiment 302)
• Virtual restriction digest
  
• Transformation of e. coli
• Analysis of a mutant hemoglobin gene
  

DNA structure and Replication (ppt file)

Transcription (ppt file)

Translation (ppt file)

Restriction digests and transformation (ppt file)

Molecular Biology Techniques (ppt file)

summary - Molecular techniques (doc file)

Virtual electophoresis lab (doc file)

pBR322 sequence (doc file)

Protein synthesis notes (doc file)

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